Detection and Illumination optics |
- Illumination: two Nikon 10X objectives, NA 0.3.
- Detection: one Nikon 25X NA 1.1 water immersion objective with switchable magnification (18x and 37X).
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Illumination
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- External laser combiner with a maximum of six wavelengths (from 405nm to 685nm), Omicron LightHUB+.
- Transmitted light illumination (LED light source) to locate the sample and acquire transmitted images.
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Light sheet specification
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- Light sheet generated by scanning of a Gaussian beam.
- Three switchable light-sheet with thicknesses (FWHM) of approximately 1.5, 2.2 and 3.3 μm for different sample sizes.
- Automatic position-specific light-sheet alignment.
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Detection camera
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- One high sensitivity Hamamatsu ORCA-Fusion USB3 cameras.
- Field of view: 18X: 800µm; 37X: 400µm
- Pixel size: 18X 347nm; 37X 173nm
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Sample part
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- Motorized XYZ sample stage with maximum X travel range of 50mm
- Multiple sample located in a open-top FEP sample chamber (multi-well).
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Sample incubation
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- Recirculating air temperature controller from 5°C above ambient temperature to 40°C.
- CO2 concentration control range 4-10%.
- O2 concentration control range 5-15%.
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